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KMID : 0613820120220040466
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Journal of Life Science
2012 Volume.22 No. 4 p.466 ~ p.471
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Genetic Diversity and Population Structure of the Scallop Patinopecten yessoensis in Korea, China, and Japan by Random Amplified Polymorphic DNA Markers
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Nam Myung-Mo
Lee Chu
Moon Tae-Seok
Huh Man-Kyu
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Abstract
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Sixty individuals of the scallop Patinopecten.yessoensis (Genus Pecten) were sampled to examine the genetic diversity and population structure of this species. Random amplified polymorphic DNA (RAPD) identified 109 genotypes and produced 79 polymorphic loci (72.8%). Total genetic diversity values (H_T) and interlocus variation in the within-population genetic diversity (H_S) were 0.254 and 0.178, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations (G_{ST}) was 0.299. This indicated that about 70.1% of the total variation was within populations. The unique loci and bands of Patinopecten.yessoensis were shown in only one population among the three countries. RAPD markers were very effective in classifying the natural population levels of P.yessoensis in Korea, China, and Japan. In addition, insights into the relative gene diversity among and within populations of P.yessoensis would be useful in breeding and for the development of strategies for animal genetic resources.
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KEYWORD
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Random amplified polymorphic DNA (RAPD), genetic diversity, Patinopecten.yessoensis
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